A microscope technique that will not allow one to see specific parts of living specimen is

Ausing stain under a light microscope

Busing a Scanning Electron Microscope

Cpassing light from a microscope through a pinhole

Dusing a fluorescent substance with a specimen under a light microscope

I know that sometimes with using a stain the specimen can be killed, but im not sure if thats always the case. Thanks in advance for your help!!