Answered

Which restriction enzyme would you use to cut the 5' end of the GFP gene?
Pst I
EcoRI
BamHI
HindIII

Verified Answer :

GinnyAnswer

Final answer:

The correct restriction enzyme to cut the 5' end of the GFP gene is HindIII, generating sticky ends for DNA fragments.


Explanation:

The restriction enzyme you would use to cut the 5' end of the GFP gene is HindIII.

HindIII is a restriction enzyme that recognizes the specific sequence 5'-AAGCTT-3' and cuts between the A and G bases, producing sticky ends.

EcoRI generates sticky or cohesive ends, and BamHI cleaves DNA at a specific 6-base sequence.


Learn more about Molecular Biology here:

https://brainly.com/question/19138651


Related Questions