DNA Profiling
NAME:
1. What is a restriction enzyme and what is its role in this process?
2. Describe the use of gel electrophoresis in DNA profiling including the role of the
electric current and fragment sizes.
3. Which way does DNA travel and why? What charge does DNA have?
4. Analyze the DNA profile to determine the paternity of the baby.
5. Identify other possible uses of DNA profiling.
Standard
•
Aratriction enzyme; enzyme that cuts the
DNA at a specific code.
There are thousands of restriction enzymes.
Each cuts DNA at a different sequence.
Some look for GGCC and cut in between the G
and C.
Every time GGCC is found in the DNA It is cut
by the restriction enzyme
DNA Code:
⚫TTATGGCCATACGGCCTT
EXAMPLE How
to cut DNA
ATAGTGGCCTATA
ATAGTGG CCTATA
Bagments
USE this restriction enzyme to "cut" (add spaces like example above) the DNA strands, and then com
boxes for each on the grid... See example below and on the grid.
Standard
CCAAGACATTATGCAGATGGCCAATAGACATTACGGCCATACCAGAGGCCCAACATGGCCAAACACACCCATCA
Mom
CCTAGACGGCCAGGCACAAGCCAGGCCATGGCCACATCAGTTAGACCGAGGCCGAATCAGGCCTTATTGCAGO
Dad
CCGAGGCCAGGGTTACCGGTATAGGCCAATTGGCCGGCATGGGCCGATACAGCCGATTGGCCATATAGGGGG
Gardener
CCGGTATTCATTACCAGGCCAAGGATACGGCAAGCAGGCCTTCGGCCAATTGGCCTTAGCACGCCGGCCAATO
Baby
CCACATCAGGTAGACCGAGGCCAATTGGCCAACCGACGGCTTAAGGCCCGACAGGCCAAAGACGGCCATATA
NAME:
1. What is a restriction enzyme and what is its role in this process?
2. Describe the use of gel electrophoresis in DNA profiling including the role of the
electric current and fragment sizes.
3. Which way does DNA travel and why? What charge does DNA have?
4. Analyze the DNA profile to determine the paternity of the baby.
5. Identify other possible uses of DNA profiling.
Standard
•
Aratriction enzyme; enzyme that cuts the
DNA at a specific code.
There are thousands of restriction enzymes.
Each cuts DNA at a different sequence.
Some look for GGCC and cut in between the G
and C.
Every time GGCC is found in the DNA It is cut
by the restriction enzyme
DNA Code:
⚫TTATGGCCATACGGCCTT
EXAMPLE How
to cut DNA
ATAGTGGCCTATA
ATAGTGG CCTATA
Bagments
USE this restriction enzyme to "cut" (add spaces like example above) the DNA strands, and then com
boxes for each on the grid... See example below and on the grid.
Standard
CCAAGACATTATGCAGATGGCCAATAGACATTACGGCCATACCAGAGGCCCAACATGGCCAAACACACCCATCA
Mom
CCTAGACGGCCAGGCACAAGCCAGGCCATGGCCACATCAGTTAGACCGAGGCCGAATCAGGCCTTATTGCAGO
Dad
CCGAGGCCAGGGTTACCGGTATAGGCCAATTGGCCGGCATGGGCCGATACAGCCGATTGGCCATATAGGGGG
Gardener
CCGGTATTCATTACCAGGCCAAGGATACGGCAAGCAGGCCTTCGGCCAATTGGCCTTAGCACGCCGGCCAATO
Baby
CCACATCAGGTAGACCGAGGCCAATTGGCCAACCGACGGCTTAAGGCCCGACAGGCCAAAGACGGCCATATA
