Which enzymes are needed to produce recombinant plasmids to be used in gene transfer?

ADNA polymerase and DNA ligase
BDNA polymerase and restriction enzyme (endonuclease)
CTranscriptase and RNA polymerase
DRestriction enzyme (endonuclease) and DNA ligase

Verified Answer :

The answer is D. For producing recombinant, first you need to cut the insert part and vector part to get the same end by using restriction enzyme. Then using DNA ligase to bind these two parts to get a new plasmids.

Answer:

D. Restriction enzyme (endonuclease) and DNA ligase

Explanation:

Hope this helps