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A lab technician runs the results of a simple PCR reaction through a gel electrophoresis to test a sample of imported hamburger meat for the presence of a DNA sequence that is known to be found in kangaroos and not in cows. What exactly will he be looking for?


A)a DNA band of a certain size on a gel


B)a green glow on a gel from the GFP marker


C)a green glow in the PCR reaction mix from GFP


D)a particular sequence of nucleotides, as determined by Sanger sequencing

Verified Answer :

C)a green glow in the PCR reaction mix from GFP

namordu

Answer: A)a DNA band of a certain size on a gel

Explanation:

In a DNA gel electrophoresis DNA fragments are separated by size (smaller fragments move faster and larger fragments move slower) and appear as discrete bands. To reveal the DNA bands the most common method is staining with Ethidium Bromide, a fluorescent compound that intercalates between base pairs in the DNA and emits light when exposed to ultraviolet light.

I’m attaching an image of a typical result of a PCR product run through gel electrophoresis. Bands in the same horizontal line have the same size.

View image namordu

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