LO60 Explain how PCR is applied in different technologies If you get a sample from a patient that wants to know if they have Covid-19 and you have a real-time PCR machine, what would you need to do to the sample before it goes in the machine considering SARS-CoV2 is a virus with an RNA-based genome.
a. Amplify the sample using CRISPR
b. Denature the RNA strand by increasing the temperature
c. Use a reverse transcriptase to get a DNA strand
d. Create a library of possible target proteins
e. Find the complementary RNA sequence of the gene of interest
a. Amplify the sample using CRISPR
b. Denature the RNA strand by increasing the temperature
c. Use a reverse transcriptase to get a DNA strand
d. Create a library of possible target proteins
e. Find the complementary RNA sequence of the gene of interest